Fig. 2: tcpΔ7 exhibits elevated cell wall pectin and reduced PG activity.
a Chemical quantification of the saccharide derivatives representing the three cell wall polysaccharides. Wall polysaccharides were extracted from the third pair of rosette leaves of 4-week-old wild-type and tcpΔ7 plants. Values are mean ± SD (n = 6 independent experiments). Statistical analysis was performed using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file. DW, dry weight. FW, fresh weight. Source data are provided as a Source Data file. b–e Immunolabeling analysis of wild type and tcpΔ7 cell wall pectin. Sections of 7-day-old seedlings were stained with LM19 to label de-methylesterified HG (b), 2F4 to label Ca2+-crosslinked de-methylesterified HG (d), and FB28 to detect cell wall cellulose. Scale bars, 100 μm. Average intensities of LM19 and 2F4, along with that of FB28, are shown in (c and e), respectively. Values are mean ± SD of 10 areas (100 μm by 100 μm) from five cotyledons. Statistical analysis was performed using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file. f Comparison of in vivo PG activity of 7-day-old wild type and tcpΔ7 seedlings. Values are mean ± SD (n = 6 independent experiments) of measured absorbance normalized to the wild type. Statistical analysis was performed using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.
