Fig. 3: TCP4 directly activates PGL1 expression.
a Heatmap showing relative transcript levels of the 68 PG genes in tcpΔ7 determined by RNA-seq. Colors represent log2 fold change in tcpΔ7 compared to the wild type. b Relative transcript abundance of PGL1 in the wild type and tcpΔ7 cotyledons determined by RT-qPCR analysis using ACTIN7 as the internal control. Values are mean ± SD (n = 3 biological replicates). Statistical analysis was performed using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file. c TCP4 binding profiles at the PGL1 locus based on ChIP-seq analysis of the wild type and 35S-MYC-mTCP4 plants. The black arrow represents the genomic region of PGL1 and the transcriptional direction. The yellow and red dots indicate the site II and CGGNCC cis-regulatory motifs recognized by TCP4, respectively. d ChIP-qPCR analysis of 7-day-old wild type and 35S-MYC-mTCP4 seedlings. Immunoprecipitated DNA using a MYC antibody was subjected to qPCR analysis. Positions of the P1 and P2 amplicons are indicated in (c). Values are mean ± SD (n = 3 biological replicates) of enrichment relative to the wild type. **, Statistical analysis was performed using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file. e Dual luciferase assay to test the effects of CIN-TCPs on the PGL1 promoter. The pPGL1:LUC-35S:REN or pPGL1m:LUC-35S:REN reporter was co-infiltrated with the 35S:TCP2/3/4/17 effectors (+) or the empty vector alone (-) into tobacco leaf epidermal cells and imaged for LUC activity. f Quantification of the LUC/REN ratio from the pPGL1:LUC-35S:REN or pPGL1m:LUC-35S:REN reporter in the presence and absence of the effectors. Values are mean ± SD (n = 4 independent experiments) normalized to the control. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparison test, and different letters above the bars indicate statistical significance at p < 0.001. Source data are provided as a Source Data file.
