Fig. 3: Spatiotemporal validation of oscillatory gene expression patterns. | Nature Communications

Fig. 3: Spatiotemporal validation of oscillatory gene expression patterns.

From: 48-Hour and 24-Hour Time-lapse Single-nucleus Transcriptomics Reveal Cell-type specific Circadian Rhythms in Arabidopsis

Fig. 3: Spatiotemporal validation of oscillatory gene expression patterns.

a–c Line plots showing the average rhythms of RBCS2B:LUC-GUS luminescence (a) AT1G66100:LUC-GUS luminescence (b) and CAB2:LUC-GUS luminescence (c) in transgenic plants (blue lines) with two biological replicates. These rhythms are compared with the pseudo-bulk expression from snRNA-seq data (orange lines), highlighting similarities and discrepancies between in vivo luminescence and sequencing data. Source data are provided as a Source Data file. d–f UMAP plots representing the specific expression patterns of RBCS2B (AT5G38420) (d), AT1G66100 (e) and CAB2 (AT1G29920) (f) within the 24 h snRNA-seq dataset. g–i Histochemical GUS assays of transgenic seedlings harboring RBCS2B:LUC-GUS (g), AT1G66100:LUC-GUS (h) and CAB2:LUC-GUS (i) reporter constructs, demonstrating cell-type-specific expression patterns. Each experiment was independently repeated three times (n = 9 samples), yielding similar results.

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