Fig. 3: Base-pairing interactions modulate viscosity of patchyDNA condensates.
From: Sequence-encoded intermolecular base pairing modulates fluidity in DNA and RNA condensates
a Representative fluorescence micrograph (n = 5 independent experiments) showing passivated beads (radius = 50 nm) embedded in a patchyDNA coacervate (white circle). b Representative time trajectory of a single bead (left) and probability distribution of bead displacement at three different lag times (right) in T-90 coacervates at 22 °C. Distributions are well-described by a Gaussian function (solid lines). c Mean squared displacement (MSD) with lag time for beads embedded in T-90 coacervates at 22 °C. Gray lines show the data from individual beads. Mean values are depicted in yellow. Black solid line is provided as a guide for slope = 1. d MSD tracks of beads (mean) in T-90 (n = 7), GC (n = 5), GTAC (n = 6), and GGCC (n = 6) condensates at 22 °C. e Similar to (d) but for beads in GGCC condensates at various temperatures (n = 5 at each temperature). f Plot showing the logarithm of viscosity (η) versus ε. The solid and open symbols denote ε was varied by changing the patch sequence or by changing the temperature respectively. Each data point denotes mean ± SD. Patch sequences and corresponding n at 22 °C T-90 (n = 7), GC (n = 5), GTAC (n = 6), and GGCC (n = 6). Temperature-dependent measurements for GGCC and GGATCC: GGCC—25 °C (n = 6), 30 °C (n = 6), 35 °C (n = 6), 40 °C (n = 6), and 45 °C (n = 8); GGATCC—25 °C (n = 5), 30 °C (n = 6), 35 °C (n = 5), 40 °C (n = 6), and 45 °C (n = 6). The dotted line is a linear fit between log(η) and ε (slope (mean ± SE) = 0.22 ± 0.01, R2 = 0.88). The color bars in (d, e, f) denote the range of ε. Error bars for some data points in f are smaller than the symbols, and thus not visible in the plot (see Supplementary Fig. 11c, d).
