Fig. 6: The structural model of cytosol-facing hVPAT.
From: Structure and mechanism of human vesicular polyamine transporter
a The AlphaFold-predicted structural model of cytosol-facing hVPAT, as viewed from the membrane. The N- and C-domains are colored cyan and yellow, respectively, and the membrane bilayer is simplified as a gray rectangle. b Overlay of the cytosol-facing model (cyan and yellow) onto the lumen-facing, spermine-bound hVPAT structure (pink), highlighting the rotational movement that the N- and C-domains undergo during the conformational transition. c The locations of spermine (pink) and the three membrane-embedded acidic protein residues (pink), E83, E171 and D255 in the spermine-bound structure, with the distances (in angstroms) between the Cα atoms specified. d The locations of the membrane-embedded E83, E171 and D255 (cyan and yellow) in the AlphaFold model, with the distances (in angstroms) between the Cα atoms indicated, underscoring the proximity of E83 to E171 or D255. The confidence scores are shown in Supplementary Fig. 16.
