Fig. 2: Mast cell deficiency prevented the demyelination and axon degeneration in nerve tissues of DPN. | Nature Communications

Fig. 2: Mast cell deficiency prevented the demyelination and axon degeneration in nerve tissues of DPN.

From: Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice

Fig. 2

a HE and TB staining were performed on patient nerve transections to reveal the neural structure. HE, hematoxylin-eosin; TB, toluidine blue. b Transmission electron microscope (TEM) images of transverse nerve sections from TLA and DPN patients. c IHC staining of NF200 was performed on the transverse and longitudinal sections of patient nerves to show the neural structure better. IHC, immunohistochemistry. d Tibial nerve tissue of DPN and TLA patients immunostained for TPSAB1 (green) and CD45 (red). e Quantification of the myelinated axon density based on TB staining in DPN and TLA nerve transections. N = 10 patients in the TLA group and N = 7 patients in the DPN group. f Quantification of demyelinated axons based on TEM analysis. N = 10 patients in the TLA group and N = 7 patients in the DPN group. g Quantification of the neurofilament 200 (NF200) immunostaining area in DPN and TLA nerve transections. N = 10 patients in the TLA group and N = 7 patients in the DPN group. h, i Quantification of CD45 and TPSAB1 expression in nerve tissue sections. N = 10 patients in the TLA group and N = 7 patients in the DPN group. j Schematic diagram of DPN induction process via intraperitoneal STZ injection. STZ, streptozotocin. Panel (j) was created in BioRender. Kong, L. (2025) https://BioRender.com/hk360ua. k Nerve conduction velocity (NCV) of DPN and control mice, based on electrophysiological test. N = 6 mice in each group. l Representative images of murine sciatic nerves co-immunostained by NF200 (green) and S100β (red). m, n Quantification of the percentage of S100β and NF200 staining area. N = 6 mice in each group. o TEM images of transverse nerve sections from DPN and control mice. p, q Quantification of intact myelin sheath and demyelinated axon density in murine sciatic nerves. N = 6 mice in each group. All data were expressed as mean ± SD in Fig. 2. Statistical significance was determined using two-tailed unpaired Student’s t tests in figure (e–i) and one-way ANOVA followed by Tukey’s post hoc analysis in figure (k, m, n, p, and q).

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