Fig. 3: Mast cell deficiency alleviated the inflammatory response in peripheral nerves following DPN induction. | Nature Communications

Fig. 3: Mast cell deficiency alleviated the inflammatory response in peripheral nerves following DPN induction.

From: Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice

Fig. 3

a Distributions of NK cells, T cells, macrophages, and neutrophils in tibial nerves of DPN and TLA patients. NK, natural killer. b Quantification of immune cell infiltration in DPN and TLA nerve transections. N = 10 patients in the TLA group and N = 7 patients in the DPN group. c, d Quantification of TNFA and IL1B expressions in DPN and TLA nerves, based on qRT-PCR experiments. N = 6 patients in each PCR group. e Gene expression of TNF-ɑ and IL-1β in tibial nerves of DPN and TLA patients displayed as UMAP plots. TNF, tumor necrosis factor; IL, interleukin. f, g Leukocyte infiltration in murine DPN nerves, and related quantification. N = 6 mice in each group. h The TNF-ɑ and IL-1β levels in sciatic nerves of DPN mice, based on enzyme-linked immunosorbent assay (ELISA) analysis. N = 6 mice in each group. i Double immunostaining of TNF-ɑ (red) and IL-1β (green) on longitudinal nerve sections of DPN mice. In panels (c, d), the ‘black dashed line’ symbol represents the median value, and the upper and lower lines of the violin plots represent the 75% and 25% quartiles, respectively. Data in panels (b, f, h) were expressed as mean ± SD. Statistical significance was determined using two-way ANOVA followed by Sidak’s post hoc analysis (b, h) and two-tailed unpaired Student’s t tests (c, d, f).

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