Fig. 4: HG environment provoked mast cell activation and degranulation. | Nature Communications

Fig. 4: HG environment provoked mast cell activation and degranulation.

From: Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice

Fig. 4

a Subclustering among the mast cell population by UMAP plots revealed 7 subpopulations. b The proportion of each subcluster in mast cell populations. c Top-ranked GO pathways of the enriched genes in DPN mast cells. d KEGG pathways enriched in DPN mast cells. e Schematic diagram of the establishment of in vitro HG environment. Panel (e) was created in BioRender. Kong, L. (2025) https://BioRender.com/1yvzy9d. f Representative immunofluorescence images of mast cell degranulation visualized by avidin (red) staining. g–i Double immunostaining of avidin (red) and CD63 (green) on mast cells and related quantification. N = 6 per group. j TEM images revealed the degranulating phenomenon in mast cells under high glucose (HG) stimulation. k–m Double immunostaining of TPSAB1 (red) and HDC (green) on mast cells and related quantification. HDC, histidine decarboxylase. N = 6 per group. (n–p) Double immunostaining of TNF-ɑ (red) and IL-1β (green) in mast cells and related quantification. N = 6 per group. Data were expressed as mean ± SD. Statistical significance was determined using two-tailed unpaired Student’s t tests.

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