Fig. 5: Memory CD8+ T cells display a stem-like phenotype in AAb+ and T1D pLNs.
From: Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset
a Frequency of CD25+ or b CD38+ cells within pLN CD8+ T cell subsets, as determined by flow cytometry. Statistical significance determined by two-sided robust ANOVA with post hoc testing using Hochberg’s multiple comparison adjustment. Boxplot represents median and interquartile range, with minima and maxima whiskers. Each data point is one pLN sample, with 52 ND, 29 AAb+, and 46 T1D samples. c Mean normalized expression of WGCNA Module 6 and effector CD8+ T cell genes of interest in pLNs. d Normalized expression of CXCR3 and TOX in pLN CD8 Tcm/Tem/Temra cells. Statistical significance determined by two-sided Wilcoxon Rank Sum test with Bonferroni correction. Dashed red line indicates the exclusive threshold (>0.5) for demarcating positive gene expression. Each point represents one cell. Boxplot information follows (a). e Frequency of pLN CD8 Tcm/Tem/Temra cells expressing permutations of CXCR3 and TOX, across disease state. P value determined by two-sided Wilcoxon rank sum test with Benjamini-Hochberg multiple test correction. Boxplot information follows (a). Each data point is one pLN sample, with 6 ND, 5 AAb+, and 7 T1D samples. f Differential surface proteins enriched in CXCR3-TOX+ cells and g CXCR3+TOX- cells compared to all other permutations of CXCR3 and TOX expression within pLN CD8 Tcm/Tem/Temra cells. Other clusters in the plot are comparators. Surface markers ranked in descending order of fold change. Statistical significance determined by Wilcoxon rank sum test on ADTs with log fold change threshold >0.1 followed by Bonferroni multiple test adjustment (adjusted p value < 0.05 denotes significance). Populations of interest highlighted by black outline. h Genes differentially expressed in CXCR3-TOX+ cells and i CXCR3 + TOX- cells, compared to the CXCR3 and TOX expression permutations, within the pLN CD8 Tcm/Tem/Temra cluster. Plot parameters are the same as in (f), but with log fold change threshold >0.25 and only genes with a p < 0.0001 are plotted. For all panels, * is p < 0.05, ** is p < 0.01, *** is p < 0.001. Source data are provided as a Source Data file.
