Fig. 6: Cytotoxic NK cells more prominent in T1D pLNs.
From: Immune perturbations in human pancreas lymphatic tissues prior to and after type 1 diabetes onset
a Frequency of CD56dimCD16+ NK cells within the pLNs, as measured by flow cytometry. Statistical significance determined by two-sided robust ANOVA with post hoc testing using Hochberg’s multiple comparison adjustment. Boxplot represents median and interquartile range, with whiskers reaching the minima and maxima. Each data point is one pLN sample, with 52 ND, 29 AAb+, and 46 T1D samples. b Representative two-parameter density plots images of NK cell subsets within the pLNs. c List of differentially expressed genes, ranked by descending log2 fold change, of genes that are significantly more expressed in T1D pLNs. Cells are from a combination of the NK and NK/ILC clusters, from the pLNs only. Gene sets to test came from the Module 6 of the WGCNA analysis, which was significantly increased in NK cell clusters in T1D, and from the “Natural killer cell mediated cytotoxicity” gene set from Kyoto Encyclopedia of Genes and Genomes (KEGG). Statistical significance tested using Wilcoxon Rank Sum Test with p-value adjustment for multiple comparisons using the Bonferroni method. d Normalized expression of GZMB and e KLRB1 within the combined NK and NK/ILC clusters in the pLNs. Statistical significance tested using two-sided Wilcoxon Rank Sum Test with p-value adjustment using the Bonferroni method. Boxplot represents median and interquartile range, with whiskers reaching the minima and maxima. f Mean normalized expression of genes significantly increased in GZMB+ and g GZMB- cells from the combined NK and NK/ILC clusters in the pLNs. P value determined by two-sided Wilcoxon Rank Sum Test with p-value adjustment using the Bonferroni method. For all panels in this figure, * is p < 0.05, ** is p < 0.01, *** is p < 0.001. Source data are provided as a Source Data file.
