Fig. 3: Characterization of Palm-M-PTH(1-14) in vitro and in vivo.
From: Prolonging parathyroid hormone analog action in vitro and in vivo through peptide lipidation
A Amino acid sequences of PTH(1-34), M-PTH(1-14) and Palm-M-PTH(1-14) and cAMP signaling responses in SGS-72 cells assessed in the presence of varying concentrations of ligand (left Ligand on), and after rinsing the cells to remove unbound ligand (right, washout). Responses were assessed as the area under the curve (AUC) of the cAMP-dependent luminescence signal vs. time plots and are plotted as a function of ligand concentration. Curves were fit to the data using a non-linear regression equation. B Curve fit parameters for the data shown in (A). Data are means ± SEM of 4 independent experiments. p-value determined by two-sided t-test (C) Blood Ca++ concentrations as a function of time after IV injection of peptide (10 nmol/kg-body weight) or vehicle in 10-week-old intact female CD1 mice. Data are means ± SEM values from six mice per group; p < 0.05 (2-way ANOVA followed by LSD test): *, vs. M-PTH(1-14); +, Palm-M-PTH(1-14) vs. PTH(1-34). Values at t = 0 represent samples collected 0.5 to 1 hr prior to injection. Source data with exact p values are provided in the Source Data File.
