Fig. 2: CTM assay H1-4 signatures showing distinct regions of profiles for different T cell states of unstimulated and untreated control, CD3/28 activation, and cryopreservation.
a–c show the H1-4 CTM assay biophysical signatures for three T cell groups at 24 h for unstimulated (control), CD3/28 activation state, and exposure to DMSO for an additional 15 min (mimicking cryopreservation), respectively. d shows unsupervised hierarchical clustering of the used to group the T cell states with respect to scaled CTM heatmap features grouped using a second unsupervised hierarchical clustering. e depicts the grouped CTM features in a Pearson’s correlation heatmap. f Selected features from the different clusters are shown here, with distinct differences in CTM features between all three T cell conditions. The box plots show the bounded 1st and 3rd quartiles with the median as center line; whiskers show the minimum and maximum values. g a principal component analysis (PCA) plot showed clear clusters of the cell conditions. All statistical mean analyses were performed using a two-tailed paired Student’s t-test with *, **, and **** denoting p-values < 0.05, 0.01, and 0.0001, respectively. The p-values are denoted below the significance symbols. Figure data included in Source Data.
