Fig. 3: Longitudinal biophysical signatures in cultured CD3+ T cells compared with flow cytometry forward scatter. | Nature Communications

Fig. 3: Longitudinal biophysical signatures in cultured CD3+ T cells compared with flow cytometry forward scatter.

From: Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes

Fig. 3: Longitudinal biophysical signatures in cultured CD3+ T cells compared with flow cytometry forward scatter.The alternative text for this image may have been generated using AI.

a describes the experiment set up with alternate-day profiling of T cells in a 14-day culture of activated T cell cultures using flow cytometry and CTM assay. b At each time point, cells were imaged using quantitative phase imaging (QPI) in the well plates with a bar scale representing 20 µm. c the frequency distribution and H1-4 histograms of the CTM assay were plotted. d Flow cytometry forward scatter plots of the corresponding cells in culture. e Interpolated forward scatter where the flow cytometry forward scatter data is interpolated into 35 bins for relative comparison with the 35 bins of CTM histogram (H4). Pearson’s correlation was performed between interpolated forward scatter data and CTM histogram, with Pearson’s coefficient shown in ρ and significance of ***, ****, and ns representing two-tailed p-value < 0.001, 0.0001, and not significant, respectively. Figure data included in Source Data.

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