Fig. 5: Elevated caspase-11 binding to LPS contributes to pyroptosis and inflammatory IL-1R signaling in PolgD257A macrophages.
From: Caspase-11 drives macrophage hyperinflammation in models of Polg-related mitochondrial disease
a Quantitation of PAO1 and lysosomes in BMDMs from (b) TEM images. Black arrows highlight outer membrane derived vesicles. N = 9 images/condition. c Quantification of capase-11 and GBP5 intensity in the area containing caspase-11, GBP5, and PAO1 from representative IF images (d). N = 10–12 IF images/genotype/condition. e ELISA analysis of IL-1 cytokines in BMDM supernatant after PAO1 or PAO1 plus anti-IL-1R blockade 2 hpi. N = 4 biological replicates/genotype/condition. f Percent BMDMs dying after PAO1 and PAO1 plus anti-IL-1R blockade from (Supplementary Fig. 9b) representative IF. N = 11–13 IF images/genotype/condition. g ELISA analysis of IL-1 cytokines in BMDM supernatant after PAO1 or PAO1 plus anti-IL-1R blockade 24 hpi. N = 4 biological replicates/genotype/condition. h ELISA analysis of inflammatory cytokines in BMDM supernatant after PAO1 or PAO1 plus anti-IL-1R blockade 24 hpi. N = 4 biological replicates/genotype/condition. Statistics: (a, c) Two-Sided Student’s t-test (e–h) Two-Way ANOVA. Error bars represent SEM. Source data are provided as a Source Data file.
