Fig. 5: Comparison of AAV-DB-3 vs AAV5 biodistribution after injection into GP in NHPs.
From: Optimized AAV capsids for basal ganglia diseases show robust potency and distribution
7.5E+10 vg of AAV-DB-3.mTFP1 or AAV5.mNeonGreen vectors were delivered to the left or right globus pallidus, respectively. A 3D model with the location of each brain slice viewed in subsequent panels (i, ii, iii, iv, v, vi, vii); Slices are 4 mm apart. Bi–vii Tile scan images showing direct fluorescence across structures. Scale bars 500 μm, inset scale bars 50 μm. C mTFP1 and mNeonGreen transcript quantification by RT-ddPCR in globus pallidus, caudate, putamen, and motor cortex (NHPs 9 and 10; Supplementary Table S1). Data shown as mean with points for each animal. Acb, accumbens; Cd, caudate; GPe, external globus pallidus; GPi, internal globus pallidus; HC, hippocampus; LGN, lateral geniculate nucleus; Pn, pons; Pu, putamen; SNc, substantia nigra pars compacta; SNr, substantia nigra pars reticulata; Th, thalamus; ac anterior commissure; ic, internal capsule. Source data are provided in the Source Data file. A used 3D reconstructions adapted from ref. 52.
