Fig. 2: Knockout of STOM affects osteoclastogenesis and osteogenesis in vivo.
From: Targeting lipid raft-related stomatin to ameliorate osteoporosis in preclinical models
A Micro-CT imaging reveals differences in bone mass between WT and STOM-/- mice; B Quantitative analysis of BMD (n = 7 biologically independent samples); C Quantitative statistics of trabecular morphological parameters, including BV, BV/TV, Tb. N, Tb. Sp and Tb. Th (n = 7 biologically independent samples); D Histological staining with H&E of mouse femur (n = 5 biologically independent samples); E, F Alizarin red and von Kossa staining of mouse femur (n = 5 biologically independent samples); G Immunofluorescence staining and quantification analysis for col1a1 in bone tissue of WT and STOM-/- mice. Fluorescence quantifies the average intensity of col1a1 across the entire image (n = 5 biologically independent samples); H Immunofluorescence staining and quantification analysis for osterix in bone tissue of WT and STOM-/- mice. Fluorescence quantifies the average intensity of osterix across the entire image (n = 5 biologically independent samples); I: ALP staining (n = 3 biologically independent samples); J Alizarin red staining (n = 3 biologically independent samples); K TRAcP staining of the distal femur and proximal tibia in mice and quantitative analysis of osteoclasts (n = 5 biologically independent samples); L Osteoclastic induction of bone marrow cells was conducted using WT and STOM-/- mice, followed by staining with TRAcP staining (n = 3 biologically independent samples). M The bone plate resorption assay was utilized to assess the osteoclastic resorption function of both WT and STOM-/- mice in vitro (n = 3 biologically independent samples). The data were represented as mean ± SD. Statistical significance was determined by two-tailed Student’s t test. WT wild type, STOM-/- STOM systemic knockout, BMD bone mineral density, BV bone volume, BV/TV trabecular volume to total bone volume ratio, Tb. N number of trabeculae, Tb. Sp bone trabecular spacing, Tb. Th trabecular thickness. Source data are provided as a Source Data file.
