Fig. 3: Systemic and osteoclast-specific knockout of STOM both mitigate OVX-induced bone loss though inhibiting osteoclasts.
From: Targeting lipid raft-related stomatin to ameliorate osteoporosis in preclinical models
A Micro-CT was used to detect changes in bone mass in WT and STOM-/- mice following OVX; B Bone parameters analysis (n = 7 biologically independent samples); C H&E staining (n = 5 biologically independent samples); D Representative images of TRAcP staining with quantification of the number of osteoclasts (n = 5 biologically independent samples); E CTX-1 levels in mouse serum (n = 5 biologically independent samples); F–H Representative Micro-CT images and quantitative analysis of BMD, BV, BV/TV, Tb. N, Tb. Th and Tb. Sp in the distal femurs (n = 7 biologically independent samples); I Representative images of distal femurs sections in each group stained with H&E (n = 5 biologically independent samples); J TRAcP staining and quantitative analysis of osteoclasts were conducted on distal femurs of STOMf/f and Ctsk-Cre; STOMf/f mice (n = 5 biologically independent samples). The data were represented as mean ± SD. Statistical significance was determined by two-tailed Student’s t test. WT wild type, STOM-/- STOM systemic knockout, BMD bone mineral density, BV bone volume, BV/TV trabecular volume to total bone volume ratio, Tb. N number of trabeculae, Tb. Sp bone trabecular spacing, Tb Th, trabecular thickness. Source data are provided as a Source Data file.
