Fig. 3: NHR-targeting antibody exhibits potent and broad neutralization against HIV-1 through bispecific antibody-mediated prepositioning.

A Neutralization potencies of iMab/D5_AR and 12 bnAbs against a panel of 119 HIV-1 pseudotyped viruses. Antibodies are grouped by color based on which of six epitopes they target (CD4-binding site, CD4bs; V1/V2 loop of gp120, V1/V2 loop; high-mannose V3 loop, High-mannose V3 loop; gp120/gp41 interface; the membrane-proximal external region, MPER; and gp41 N-heptad repeat, NHR). The black dotted line indicates the limit of quantification (50 μg/mL). Error bars indicate median +/- interquartile range. Neutralization potencies of antibodies, apart from iMab/D5_AR and 10E8v4, were obtained from published studies curated in the Los Alamos HIV database (shown in gray background), CATNAP. Source data is provided as a source data file. Each dot represents the average neutralization of technical replicates for each pseudotyped virus. B Neutralization potency vs. breadth curve against 119 HIV-1 pseudotyped viruses. Antibodies were grouped by the epitope targeted (colors in key). Breadth was defined as non-protective when IC₈₀ > 5 μg/mL. The blue dotted line indicates IC₈₀ of 5 μg/mL. Source data ARE provided as a source data file.