Fig. 1: Experimental procedure of the phospho-, acetyl-, and global proteomic analysis of human skeletal muscle biopsy tissues. | Nature Communications

Fig. 1: Experimental procedure of the phospho-, acetyl-, and global proteomic analysis of human skeletal muscle biopsy tissues.

From: Aerobic and resistance exercise-regulated phosphoproteome and acetylproteome modifications in human skeletal muscle

Fig. 1: Experimental procedure of the phospho-, acetyl-, and global proteomic analysis of human skeletal muscle biopsy tissues.

After trypsin digest, peptides were isobarically labeled and combined into two tubes. One tube was acetyl enriched and the other tube was fractionated by high-performance liquid chromatography (HPLC). Of these, two distinct fractions were recombined, resulting in 48 tubes, of which a small portion was used for global proteomics analysis. Four tubes with distinct fractions were combined again, resulting in 12 tubes, which were used for phospho-enrichment. Mass spectra were measured on all samples using a Thermo Fusion Tribrid coupled to Ultimate 3000 HPLC. Created in BioRender. Pataky, M. (2025).

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