Fig. 5: hnRNPL regulates PIK3CB transcription to enhance glycolysis.
From: hnRNPL phase separation activates PIK3CB transcription and promotes glycolysis in ovarian cancer
a Spearman’s correlation analysis between mRNA levels of hnRNPL and PIK3CB according to the GEO ovarian cancer cohorts. b Schematic diagram of the luciferase reporters carrying wild-type or mutated PIK3CB promoter. c Analysis of luciferase activities of the reporters carrying wild-type or mutated PIK3CB promoters normalized to renilla luciferase activity in ovarian cancer cells with knockdown of hnRNPL. d Effect of hnRNPL loss on RNA Pol II enrichment at the promoter of PIK3CB. e Effect of wild-type or mutated hnRNPL overexpression on the PIK3CB mRNA level. f ChIP assays were performed to evaluate the enrichment of wild-type or mutated hnRNPL at the PIK3CB promoter in OVCAR3 cells by using the antibody specific to hnRNPL. g Schematic diagram of PI3K-AKT regulated glycolytic signaling pathway. h Western blot of PIK3CB and PI3K-AKT signaling pathway-associated proteins in ovarian cancer cells upon overexpression of wild-type or mutated hnRNPL. i Glucose content and lactate production in the culture medium were monitored in ovarian cancer cells upon overexpression of wild-type or mutated hnRNPL. j ECAR levels were determined in wild-type ovarian cancer cells and ovarian cancer cells with overexpression of hnRNPL variants of WT, IDR1delG, IDR2delP by the Seahorse assays. Data are shown as means ± S.D. p value was calculated by one-way ANOVA test with multiple comparisons (c, e, f, i, j) and unpaired two-sided Student’s t-test (d). Data are representative of at least three independent experiments (c–f, h–j). Source data are provided as a Source Data file.
