Fig. 2: Single-molecule sensing of L-cysteine (Cys) and L-homocysteine (Hcy) by ferritin nanopore with the assistance of Cu2+.
a Schematic of ferritin nanopore sensor. The lipid bilayer divides the flow cell into cis (grounded) and trans chambers, and the testing solution is added into the trans chamber for detection. b Current trace at +40 mV in the presence of L-Cys (80 µM) and Cu2+ (80 µM) in the trans side. c Current trace at +40 mV in the presence of L-Hcy (80 µM) and Cu2+ (80 µM) in the trans side. d, e Scatter plot and histogram showing the dwell time and ΔI/I0 of blockage signals in Cys (n = 174) and Hcy (n = 201) sensing.
