Fig. 3: Activation of TGR5 decreases inflammation and leptin signaling suppressor in the NTS. | Nature Communications

Fig. 3: Activation of TGR5 decreases inflammation and leptin signaling suppressor in the NTS.

From: Pharmacological and physiological activation of TGR5 in the NTS lowers food intake by enhancing leptin-STAT3 signaling

Fig. 3

Relative NTS Cd68 (a), Il6 (b), Tnfa (c), LepRb (d), Ptp1b (e), and Socs3 (f) mRNA expression of NTS CMC (n = 8) or CCDC (n = 6) chow rats and NTS CMC (n = 8 for a–d; n = 7 for e, f) or CCDC (n = 8 for a–d; n = 7 for e, f) HF rats, measured 24 h after food was given back following 5 min acute infusion. g Representative images (scale bar: 100 μm; 25 μm for the digital zoomed-in images) showing FISH for Tgr5, Aif1, and DAPI or LepR, Aif1, and DAPI in the NTS of HF rats 30 min after CMC 5 min acute infusion. Digital zooms of the boxed regions are shown to the left. Arrowheads indicate cells co-expressing Tgr5 or LepR and Aif1. h Left: Proportion of Aif1+ microglia in the NTS that are Tgr5+ or Tgr5- and LepR+ or LepR- (pie chart). Right: Percentage of Aif1+ microglia expressing Tgr5 or LepR in the NTS (n = 3 per group). i Representative images (scale bar: 100 μm; 25 μm for the digital zoomed-in images) showing FISH for Tgr5, Rbfox3, and DAPI or LepR, Rbfox3, and DAPI in the NTS of HF rats 30 min after CMC 5 min acute infusion. Digital zooms of the boxed regions are shown to the left. Arrowheads indicate cells co-expressing Tgr5 or LepR and Rbfox3. j Left: Proportion of Rbfox3+ neurons of NTS that are Tgr5+ or Tgr5- and Lepr+ or Lepr- (pie chart). Right: Percentage of Rbfox3+ neurons expressing Tgr5 or LepR of NTS (n = 4 per group). k Representative images (scale bar: 100 μm; 25 μm for the digital zoomed-in images) showing FISH for Tgr5, LepR, and DAPI in the NTS of HF rats 30 min after CMC 5 min acute infusion. Digital zooms of the boxed regions are shown to the left. Arrowheads indicate Tgr5 expressing cells that are either LepR+ (top) or LepR- (bottom). l Left: Proportion of Tgr5+ cells in the NTS that are Lepr+ or Lepr- (pie chart). Right: Percentage of Tgr5+ cells that are Lepr- or Lepr+ in the NTS (n = 4). a–f P values were calculated by one-way ANOVA with Tukey’s multiple comparison; h, j P values were calculated by two-tailed t tests; l P values were calculated by two-tailed paired t tests. Data presented as mean ± SEM.

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