Fig. 1: LCI20 subcellular localization, characterization and complementation of a lci20 insertional mutant.

a Subcellular localization of LCI20 protein fused with the Venus fluorescent reporter. False colours were used to represent Venus (green) and chlorophyll (magenta) fluorescence signals. Scale bar, 5 µm. The localization of LCI20-Venus in the chloroplast envelope was confirmed by three independent experiments. b Genomic structure of LCI20 gene and insertion site of the paromomycin resistance cassette in the CLiP lci20 mutant. Gray boxes at both extremities represent the 5’ and 3’UTR, respectively. Exons are colored blue, and the position of cassette insertion is indicated by red arrows. c Immunoblot analysis using anti-LCI20 antibodies. Uncropped immunoblots are provided as a Source Data file. Due to the limited amount of LCI20 antibodies, this immunoblot was repeated twice with a similar result. Photoautotrophic growth of lci20, CC4533 (i.e. WT in this study all throughout) and complemented lines (C1, C2) on agar plates kept under various CO₂ regimes. Cells were grown photo-autotrophically in liquid culture under air CO₂ level (d), or air supplemented with 2% CO₂ (e) prior to spot testing. Images were taken 3 days (H-CO₂ and L-CO₂) or 5 days (VL-CO₂) after growing under 80 μmol photons m⁻² s⁻¹.