Fig. 6: Metabolomic analysis shows increased accumulation of malate and glutamate in lci20 during a transition from H-CO2 to VL-CO2 under photorespiratory but not under non-photorespiratory conditions.

Accumulation of intracellular glutamic acid and malic acid in cells grown (a) under H-CO2, (b) or after transition from H-CO2 to L-CO2 for 20 h, (c) after transition from H-CO2 to VL-CO2 under 21% O2 or (d) under 2% O2 for 20 h. Bars show the average and dots show data from independent biological replicates (n = 8 ± SD). Asterisks represent statistically significant difference compared to the wild-type strain CC4533 (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001) using two-way ANOVA. A.U., arbitrary unit; ns, not significant.