Fig. 7: Structure of the variediene synthae (EvVS) cyclase domain. | Nature Communications

Fig. 7: Structure of the variediene synthae (EvVS) cyclase domain.

From: Structure of bifunctional variediene synthase yields unique insight on biosynthetic diterpene assembly and cyclization

Fig. 7

A Overlay of the cryo-EM structure of the cyclase domain from Map 3 (magenta), the AlphaFold3 structure prediction (purple), and the crystal structure of FgGS (yellow). B Close-up view of the active site of the EvVS cyclase domain. Density for the diphosphate group is shown by a light blue surface for cyclase Map 3 (EMD-47457, contour level 0.090). C The inorganic pyrophosphate anion in the active site of FgGS (PDB 6VYD) binds similarly to the diphosphate group in the EvVS cyclase active site shown in (B). Also bound in the FgGS active site is the benzyl triethylammonium cation (BTAC).

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