Fig. 2: Mitochondrial fusion-deficient mutants exhibit iron dyshomeostasis.
From: Deficits in mitochondrial dynamics and iron balance result in templated insertions
a Examples of upregulated genes of the iron regulon in mitochondrial fusion-deficient mutants with three biological replicates of each strain, logFC - log fold-change. b FIT2pr-GFP fluorescence signal indicating iron regulon activation in arbitrary units (mean ± SD, n = 3 biological replicates of 10,000 events each, F = 39.53, W = 3612, df=3). P values were determined using two-tailed Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test. Exact cell counts are provided in Supplementary Data 4. c Intensity of iron uptake proteins (Ftr1-GFP, Fet3-GFP, and Arn1-GFP) in wild-type and mgm1Δ cells. Exposure time was the same in wild-type and mgm1Δ cells. Scale bars represent 5 µm. Experiments were repeated independently three times with similar results. d Percentage of cells containing nuclear Rps2-GFP foci and representative images from mgm1Δ cells. P values determined by two-tailed χ2 test, n = 1607, 1052, 1209, and 1000 total cells, respectively. Scale bar represents 5 µm. e Localization of ISC biogenesis proteins (Yfh1-GFP, Nfs1-GFP, Acp1-GFP, and Grx5-GFP) with MitoTracker Red mitochondrial staining in wild-type and mgm1Δ cells. Scale bar represents 5 µm. Experiments were repeated independently three times with similar results. f Percentage of cycling cells (excluding G0/G1 phase) with spontaneous Rad52-GFP foci and representative image from mgm1Δ cells. P values determined by two-tailed χ2 test, n = 1558, 1040, 1064, and 1058 total cycling cells, respectively. Scale bar represents 5 µm. Source data are provided as a Source Data file.
