Fig. 5: Iron depletion or overload leads to templated insertions.
From: Deficits in mitochondrial dynamics and iron balance result in templated insertions
a, b Frequency and types of inserted DNA at DSBs. “+Fe” indicates treatment with 3 mM ammonium iron (II) sulfate for 6 h prior to plating on galactose. “+BPS” indicates treatment with 250 mM BPS iron chelator overnight prior to plating on galactose. P values were determined using two-tailed χ2 test. n – number of NHEJ products tested shown in Supplementary Data 1–3. c Percentage of cells containing Rps2-GFP nuclear foci. “+Fe” indicates treatment with 3 mM ammonium iron (II) sulfate for 6 h prior to imaging. P values determined by two-tailed χ2 test, n = 1607, 1120, 1009 total cells, respectively. d Frequency of NUMTs at DSBs. “+BPS” indicates treatment with 250 mM BPS iron chelator overnight prior to plating on galactose. P values were determined using two-tailed χ2 test. n – number of NHEJ products tested shown in Supplementary Data 1–3. e Distribution of insertion donor DNA in indicated mutants. n – number of independent insertion donor DNA. Source data are provided as a Source Data file.
