Fig. 3: Dynamic cell-cell communication patterns mediated by ligand-receptor interactions upon IR.
a UMAP visualization of cytokine scores for each cell type in BM cells. b Violin plots showing the distribution of cytokine scores across different cell types in BM cells. c Dotplot showing the expression profiles of cytokines across different cell types. Dot color indicates the average expression levels, and dot size indicates the fraction of expressing cells. d Violin plots showing representative cytokine expression changes in basophils and T/NKs at D0 and at D3 after 6.5 Gy of IR. e Fold change in OSM and IL6 expression levels in basophils, and fold change in CCL5 expression levels in T cells, at D0 and at D3 after 6.5 Gy of IR. n = 3 or 4 biological replicates per group. Data shown are mean ± SEM. f Dotplot showing the enriched LR pairs between HSPC subpopulations and niche cells. Dot color indicates the fold change (FC) of enrichment, and dot size indicates the P value of enrichment. g Two clusters of LR interactions with distinct temporal intensity patterns (left) were observed between different HSPC subpopulations and niche cells (right). h Heatmap showing the intensities of specific LR pairs across different time points after IR. “Cell to” indicates the cell type highly expressing the receptor in the corresponding ligand-receptor. “Cell from” indicates the cell type highly expressing the ligand in the corresponding LR. i Fold change in BMP4 expression levels in CD45−VE-CAD⁺ endothelial cells and CD45−LEPR⁺ stromal cells at D0 and D1 after 6.5 Gy of IR. j Fold change in pSMAD1/5 expression levels in HSCs at D0 and D1 after 6.5 Gy irradiation. n = 4 biological repeats per group. Data shown are mean ± SEM. Statistical analyses were conducted using unpaired two-sided Student’s t tests (e, i, j). Source data are provided as a Source Data file.
