Fig. 4: Characterization and performance of BX004-A. | Nature Communications

Fig. 4: Characterization and performance of BX004-A.

From: Phage therapy with nebulized cocktail BX004-A for chronic Pseudomonas aeruginosa infections in cystic fibrosis: a randomized first-in-human trial

Fig. 4: Characterization and performance of BX004-A.The alternative text for this image may have been generated using AI.

A Schematic illustration of P. aeruginosa genes involved in phage life cycle, as discovered by our analysis. Phage are color-coded as depicted on the top right legend. The arrows by the gene names represent the average transcript abundance rank of the gene in sputum of pwCF (→: percentile 0.33–0.66, ↑: percentile > 0.66). This graphic was created in BioRender. Weiner, I. (https://BioRender.com/9qfpmc8). B Tree visualization of genomic distances between RefSeq P. aeruginosa cluster representatives and the 143 clinical isolates in our panel. Strain sensitivity to BX004-A is given in the outer circles. C Subpopulation analysis of BX004-A host range on strains containing each of the prevalent P. aeruginosa aPDS (found in at least 5% of strains). Enrichment tests – using Fisher’s exact test with Benjamini Hochberg correction, returned p > 0.05 in all cases. D, E Antibiofilm efficacy, as measured by ATP quantitation (D) and biomass staining (E). The two different strains used in this experiment are color-coded as depicted on the top right corner of panel D. Data shown for each condition is average ± SE across all available biological repeats. Sample sizes per condition alongside all other source data are provided as a Source Data file.

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