Fig. 10: Meiosis resumption benefits from increased meiotic SGs disassembly duration after heat relief.
From: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
A Top, schematic of experimental design. Yellow block: 25 °C or 42 °C treatment; black tick: β-estradiol added; red ticks: sample collection. Middle, representative cells showing 1 nucleus (1 N, before meiotic division), 2 nuclei (2 N, finished meiosis-I), 4 nuclei (4 N, finished meiosis-II), and >4 nuclei (>4 N, aberrant chromosome segregation). Green: GFP-Rim4, magenta: nuclei (DAPI). Bottom, percentage of 1 N, 2 N, 4 N and >4 N cells. Lines: mean, shades: SE. Blue: WT Hsp104, 25 °C; red: WT Hsp104, 42 °C; green: ∆hsp104, 25 °C; orange: ∆hsp104, 42 °C. N (cells, from 12 to 24 h) = (blue) 142, 179, 392, 402, 199, 114, 291, 141, 137, 283, 247, 233, 225, 209, 220, 180, 391, 296, 174, 202, 366, 347, 143, 342, 179; (red) 104, 161, 192, 153, 154, 121, 163, 151, 143, 120, 160, 203, 226, 182, 249, 186, 171, 112, 183, 179, 166, 188, 169, 191; (green) 166, 135, 161, 114, 149, 202, 120, 142, 121, 232, 85, 132, 188, 182, 169, 159, 134, 109, 106, 141, 119, 153, 80, 184, 98; (orange) 121, 91, 123, 148, 146, 128, 143, 123, 159, 133, 191, 169, 210, 262, 236, 153, 234, 270, 207, 188, 205, 277, 220, 320. B Top, schematic of experimental design. Yellow block: 1-h treatment of the indicated temperature. Arrow: β-estradiol added. Bottom, sporulation efficiency of the WT Hsp104 or ∆hsp104 cells. N (repeats) = 18, 12, 9, 6, 15, 12. Dots: repeats; red lines: mean ± SE. Unpaired two-sided Mann–Whitney test. p-values: a: 4.62e-8 (***); b: 0.7756 (ns); c: 5.75e-8 (***). C Sporulation efficiency of cells with 1-h heat treatment at indicated time points. Black: WT Hsp104; blue: ∆hsp104. Dashed lines: mean sporulation efficiency of WT Hsp104 (black) and ∆hsp104 (blue) cells with no treatment (NT). N (repeats) = (NT, 12 h) 6, (others) 3. Data are shown as mean ± SE. D GFP-Rim4 foci number per cell. N (cells) = 181, 176, 176, 154. Box and whisker: Center line, median; box limits, upper and lower quartiles; whiskers, minimum and maximum range. Unpaired two-sided Mann–Whitney test. p = 5.58e-36 (***). E Dual-function model of meiotic SGs. Scale bars are 5 µm.
