Fig. 9: Meiotic SGs halt gametogenesis upon heat shock.
From: Rim4-seeded stress granules connect temperature sensing to meiotic regulation
A Top, schematic of the experimental setup. Bottom, sporulation efficiency represented by tetrads percentage (black line) and SG formation represented by SD of GFP-Rim4 intensity (purple line, from Fig. 1J) plotted against temperature. Sporulation sample N (repeats) = 15, 6, 12, 12. Data are shown as mean ± SE. B–D FM of the cell expressing GFP-Rim4(PM) and mScarlet-Pab1 at 25 °C. B Top, schematic of GFP-Rim4(PM). PMIst2 (blue): plasma membrane (PM) binding domain derived from Ist2. Bottom, Representative FM images. Yellow boxes and lines are as described in Fig. 1F. Scale bars are 5 µm. C Average foci number per cell. Red dashed line: WT foci number per cell under 42 °C (data from Fig. 1K). N (cell) = 134, 131. Box and whisker: center line, median; box limits, upper and lower quartiles; whiskers, minimum and maximum range. Unpaired two-sided Mann–Whitney test. p = 6.64e-41 (***). D Percentage of Pab1/Rim4 (beige) and Rim4/Pab1 (brown) co-localization per cell. Dashed lines, light brown: Pab1/Rim4; dark brown: Rim4/Pab1, from Rim4(WT) strains under heat shock in Fig. 1G. Dots: cells analyzed; red lines: mean ± SE. N (cells) = 114, 113. E Top, schematic of experimental setup. Bottom, sporulation efficiency of indicated strains. N (repeats) = 9. Dots: repeats; red lines: mean ± SE. Unpaired two-sided Mann–Whitney test. p-values: a: 4.11e-5 (***); b: 4.11e-5 (***).
