Fig. 1: Combination of zyp1 and recq4 massively increases meiotic crossover frequency.
From: Maximizing meiotic crossover rates reveals the map of Crossover Potential
a Schematic representation of the experimental design. Col/Ler hybrid plants, either wild-type or carrying mutations, were crossed with wild-type Col in both directions. The resulting plant populations (BC1) were sequenced to score the COs transmitted by the female and male gametes of the hybrids. Created in BioRender. Lian, Q. (https://BioRender.com/m7vjhfg). b The number of COs per female and male gametes for each genotype. Each point represents the number of COs in one BC1/gamete. Circles and triangles are females and males, respectively. A black line indicates the mean and its value is specified at the top of the graph. The BC1/gamete population size (n) is indicated for each genotype. Two-sided Mann-Whitney test was used to evaluate the differences in CO numbers between female and male meiosis (***: < 10−3). See also Figure S5 for chromosome per chromosome analysis (c) Representative transmitted chromatid sets are shown for selected samples. The genotype and the name of the sample are specified. Red and Blue indicate Col and Ler genotypes, a transition marking a crossover. White points indicate centromeres. d The average number of COs per F2 sample (for recq4 figl1, yellow) or pseudo-F2 (sum of female and male averages, for all other genotypes).
