Fig. 6: OsLYK11 is conserved in indica but not in japonica rice. | Nature Communications

Fig. 6: OsLYK11 is conserved in indica but not in japonica rice.

From: Several groups of LysM-RLKs are involved in symbiotic signal perception and arbuscular mycorrhiza establishment

Fig. 6

a Partial sequence alignment of OsLYK11 from the indica rice cultivar Xihui18 and the japonica rice cultivars Nipponbare and Kitaake, showing the natural 1 bp deletion in the japonica cultivars. b Image of epidermal cells from a N. benthamiana leaf expressing Xihui18 OsLYK11-YFP (OsLYK11). Scale bar represents 20 µm. c Affinity of OsLYK11 for LCO-V(C18:1,NMe,S). Scatchard plot of a cold saturation experiment using membrane fractions containing OsLYK11 and a range of LCO-V(C18:1,NMe,S) concentrations as competitor. The plot is representative of experiments performed with two independent batches of membrane fractions. d Affinity of OsLYK11 for short-chain CO. Saturation experiments on 10 µg of membrane proteins containing OsLYK11 using a range of concentrations of CO5-biot. WB performed using sequentially anti-GFP antibodies and streptavidin on the same membrane. The arrowhead indicates the position of OsLYK11-YFP, whereas * indicates an N. benthamiana endogenously biotinylated protein. e CO5-biot binding to 10 µg of N. benthamiana membrane proteins containing OsLYK11 or OSNFR5/OsMYR1. f Position of the frameshift insertion in Xihui18 Oslyk11-1. Details are shown in Supplementary Fig. 13b. g Detailed analysis of AMF structures at 5 wpi in Oslyk11-1 or WT Xihui18 plants. n = biologically independent plants examined over two independent experiments. h Detailed analysis of AMF structures at 5 wpi in Nipponbare or Kitaake plants WT, containing the empty vector (WTL) or containing Xihui18 OsLYK11. n = biologically independent plants from two independent transgenic lines and one WT line from one experiment. A second replicate is shown in Supplementary Fig. 13c. The box edges represent the 0.25 and 0.75 quantiles, the center line indicates the median value, and the whiskers show the range from the minimum to the maximum values, excluding the outliers in (g, h). Statistical differences (p < 0.05) were calculated using a Van der Waerden test in (g, h) (for each structure category).

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