Fig. 3: FABP7 increased basal ECAR and inhibition of glycolysis by 2DG reduced the effect of FABP7 in monocytes.
Monocytes were treated with FABP7 and FABP4 for 48 h, and a seahorse assay was performed. a–e Graphs (mean ± SD) indicate (a) OCR and (b) ECAR in monocytes treated with FABP7, bar diagram represents (c) basal respiration, (d) ATP-linked respiration/ATP production and (e) basal ECAR (n = 8 for control and FABP7 and n = 5 for LPS, biological replicates). f, g Graphs (mean ± SD) indicate OCR and ECAR in monocytes treated with FABP4 (n = 8 for control and FABP4 and n = 5 for LPS, biological replicates). h Monocytes were treated with FABP7 in the presence or absence of 2DG for 48 h, and flow cytometry was performed, data is represented as bars (mean ± SD, n = 24) indicating the percentage of cell population gated from CD14+ cells, and (i) zebra plot showing expression of IL-1β. j Effect of FABP7 on ROS production in monocytes of HC. ROS assay was performed in Monocytes treated with FABP7 for 6 h using flow cytometry, and bars represent MFI of ROS positive cells (mean ± SD, n = 5), a representative histogram is also shown for ROS. k Mitochondrial superoxide was determined using flow cytometry after treating the HC monocytes with FABP7 for 48 h, the bar (mean ± SD) represents MFI of MitoSox positive cells (n = 6), and a representative histogram is also shown for MitoSox. One-way ANOVA was performed following correction with Dunnett’s multiple comparison test for (c–e, j, and k), and correction with Šidák multiple comparison test for (h). P-values are shown above the comparisons. OCR Oxygen consumption rate, ECAR Extracellular acidification rate, ROS reactive oxygen species.
