Fig. 4: Genetic lineage tracing of c-kit+ progenitors converting to brown adipocytes during fat homeostasis. | Nature Communications

Fig. 4: Genetic lineage tracing of c-kit+ progenitors converting to brown adipocytes during fat homeostasis.

From: Commitment of adipose-resident c-kit+ progenitors to brown adipocytes contributes to adipose tissue homeostasis and remodeling

Fig. 4: Genetic lineage tracing of c-kit+ progenitors converting to brown adipocytes during fat homeostasis.

A Schematic depicting the experimental procedures. Ten-week-old Kit-CreER; Rosa26-RFP mice were administered with Tam, and different adipose tissues were collected at 10 and 40 weeks after Tam treatment, respectively, for analysis. B–G Classic interscapular brown adipose tissue (iBAT), iBAT that interfaces with interscapular white adipose tissue (iBAT/iWAT), and perigonadal white adipose tissue (pgWAT) were collected for immunofluorescence staining of the red fluorescent protein (RFP) lineage marker and the uncoupling protein 1 (UCP1) thermogenic marker, n = 6 mice/group. At 10 weeks post-Tam treatment, RFP+UCP1+ cells were observed in iBAT (B) and iBAT/iWAT (C), whereas few RFP+UCP+ cells were observed in pgWAT (D). At 40 weeks post-Tam treatment, more RFP+UCP1+ cells were observed in iBAT (E) and iBAT/iWAT (F) but not in pgWAT (G). Scale bar = 100 μm.

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