Fig. 7: MHFD reduces the proportion of M+N+ progenitors and their descendant β cells before birth while inducing postnatal β-cells proliferation. | Nature Communications

Fig. 7: MHFD reduces the proportion of M+N+ progenitors and their descendant β cells before birth while inducing postnatal β-cells proliferation.

From: Pancreatic islet β-cell subtypes are derived from biochemically-distinct and nutritionally-regulated islet progenitors

Fig. 7

a The experimental design. b A few DEGs in Ngn3+ cells with mHFD or control diet (CD) treatment. Shown are the levels of expression normalized against that of CD-treated cells. c, d IF staining showing tdT (red) and Ki67 (white) expression in β cells (green) in MNT mice with CD or mHFD exposure immediately after birth (P1). A merge and the three single channels are presented. White arrows, tdT-Ki67+ β cells. Yellow arrows, tdT+Ki67+ β cells. 9 controls and 12 mHFD mice were examined, with similar results. Bar = 20 μm. e, f The proportion of tdT+ β cells (mean + SEM, (e) and the proliferation rate (mean + SEM, (f) (ki67 expression) of tdT+ and tdT- β-cell subtypes. P-values were calculated using unpaired t-tests with two-sided type-two errors. “n” refers to the number of mice examined (9 controls and 11 mHFD mice).

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