Fig. 5: Differential splicing analysis and the detection of alternative splicing regulated by splicing factors.
From: Single cell and spatial alternative splicing analysis with Nanopore long read sequencing
A The principle of generalized likelihood ratio test to identify differentially expressed isoforms. The mean change vs. variance change of ψ for all significant meta splicing sites identified in Jurkat (B) and stimulated Jurkat cells (C) after knock-out of splicing factors. Each point is a significant meta-splicing site labeled by its gene identity. Meta splicing sites with too low mean or variance change are not labeled. D Correspondence of significant meta-splicing sites between original and stimulated Jurkat cells. The line represents the fitted linear regression; shaded areas indicate the 95% confidence interval around the fit. After stimulation, there is a significant change of gene expression and alternative splicing in Jurkat cells, but the regulation of splicing factors keeps the same direction. Source data are provided in https://doi.org/10.5281/zenodo.15320816.
