Fig. 7: Longcell application on the VISIUM sequencing of a mouse olfactory bulb slice.
From: Single cell and spatial alternative splicing analysis with Nanopore long read sequencing
A The spatial plot for the VISIUM slice, each spot is colored by the layer identification. B ϕ vs. \(\bar{\psi }\) distribution for alternative spliced exons, color indicates the confidence interval of ϕ. C The mean change vs. variance change of ψ for all significant meta-sites which are alternatively spliced across different layers. The point size indicates the significance after FDR control, while the color indicates in which layer this meta-site is alternatively spliced. D The ψ distribution for exon 3 of Plp1, which has the highest ϕ and shows a bimodal distribution, indicating a low inter-cell heterogeneity. E Spatial plot of spots in the slice of mouse olfactory bulb. The spots are colored by ψ for exon 3 of Plp1. Cells which have low expression (<3) of this gene and could not give a confident ψ estimation are the smallest points colored in gray. The gene expression for each spot is shown by the point size, while the ψ estimation is shown by the color gradient. F, G The alternative splicing for Plp1 in different layers. H The ψ distribution for exon 4 of Mapre3, which has a relatively high ϕ and show a bimodal distribution, indicating a high inter-cell heterogeneity. I Spatial plot of spots in the slice of mouse olfactory bulb. The spots are colored by ψ for exon 4 of Mapre3. J, K The alternative splicing for Mapre3 in different layers. Source data are provided in https://doi.org/10.5281/zenodo.15320816.
