Fig. 3: OPA1-deficient NP cells show dysregulated bioenergetics.
From: The loss of OPA1 accelerates intervertebral disc degeneration and osteoarthritis in aged mice
A, B ECAR and oxygen OCR traces from NP cells transduced with ShCtrl and ShOpa1 in the absence of exogenous glucose and after sequential addition of 10 mM glucose followed by oligomycin and rotenone plus myxothiazol. C Mitochodrial OCR measurement between ShCtrl and ShOpa1 under basal, glucose, and oligomycin addition (D) ATP production rate from glycolytic and oxidative metabolism calculated from traces shown in (A) and (B). E, F ECAR and OCR traces in the absence of exogenous glucose and after sequential addition of 10 mM glucose, followed by rotenone plus myxothiazol, and finally monensin plus FCCP. G Proton production rate (PPR) of NP cells calculated from traces shown in (D) and (E). Data represent 3-4 independent experiments, each with four replicates/group. Data is shown mean ± SEM. t-test or Mann–Whitney two-tailed test or one-way ANOVA was used as appropriate (C, D, G). Source data are provided as a Source Data file.
