Fig. 9: The loss of OPA1 significantly alters NP tissue metabolic balance.
From: The loss of OPA1 accelerates intervertebral disc degeneration and osteoarthritis in aged mice
A WT and Opa1cKO transcriptomic clustering profiles using Principal Component Analysis (PCA). B, C Volcano plot and Hierarchical clustering of differentially expressed genes (DEGs) with FDR < 0.05%. D The CompBio biological map generated from downregulated DEGs FDR < 0.05%. The size of the sphere corresponds to its enrichment score, while the thickness of the lines connecting themes represents the number of genes shared between them. E–K Downregulated themes and related DEGs associated with hypoxia, glycolysis, lysophosphatidic acid (LPA), one-carbon metabolism, procollagen dioxygenase, and autophagy/mitophagy in Opa1cKO mice were shown with CompBio entity score. L–N’ The gene set enrichment analysis (GSEA) of mitochondrial pathways (MitoPathways 3.0) of Opa1cKO downregulated DEGs showing ETC complex IV subunits, OXPHOS and branched chain amino acids metabolism. MitoPathways3.0 was employed to calculate gene set enrichment using the log2 values for pre-ranking the genes and gene set size filters (min=15 and max = 500). A negative enrichment score (ES) indicates gene set enrichment at the bottom of the ranked list. Source data are provided as a Source Data file.
