Fig. 7: HNP1 affects the metabolism of A. baumannii while exhibiting minimal effects on biofilm-related gene transcription.

A PCA plot of RNA-seq data from A. baumannii WT and ΔOmpA strains treated with/without 8 μM HNP1 (n = 3 for each group). B Hierarchically clustered heatmap representing sample-to-sample distances calculated using Poisson distances, illustrating the overall similarities between samples. C Venn diagrams showing the overlap of DEGs between the three comparisons: WT + HNP1 vs. WT, ΔOmpA vs. WT, and ΔOmpA + HNP1 vs. ΔOmpA. The upper panel shows overlaps of upregulated genes, while the lower panel shows overlaps of downregulated genes. D Volcano plots depicting the DEGs from the DESeq2 analysis for the three comparisons. Genes significantly upregulated are shown in red, while significantly downregulated genes are shown in blue. Non-significant genes are indicated in gray. E COG category distribution of DEGs. The upregulated and downregulated genes in each comparison are categorized by their COG functional groups, with blue representing downregulated genes and orange representing upregulated genes. F Heatmap showing the expression of nine-groups of biofilm-related genes across the four experimental groups. The upper panel displays the mean log2 fold change relative to WT for each gene, and the lower panel shows the count per million (CPM) expression values for the same genes. See also Supplementary Figs. S12 and S13, Supplementary Data.