Fig. 1: LER and COL potentiate CAS against A. fumigatus.
a Metabolic activity (%) of A. fumigatus with the four compounds identified as potentiators of CAS activity. A. fumigatus was grown in liquid minimal medium (MM) with 10% of Alamar blue using 96-well plates at 37 °C in the presence of different concentrations of each drug combined with 0.2 μg/mL of CAS. After 48 h, the metabolic activity (%) was assessed by reading fluorescence at a wavelength of 570 nm excitation/590 nm emission. The results represent the average of two independent experiments performed in technical duplicate. b, c Structures of Lercanidipine (LER) and Colistin (COL). d, e Fungicidal activities of COL + CAS and LER + CAS against A. fumigatus. Conidia (1 × 104/mL) were incubated with 0.2 μg/mL of CAS and different concentrations of LER and COL in 96-well plates. After 48 h at 37 °C, the plates were centrifuged, the supernatants with the drugs were removed, and the cells were plated on solid MM and incubated for 48 h at 37 °C. The results represent the average of three independent experiments ± standard deviation (SD). The data was statistically analyzed by the ordinary one-way ANOVA and Dunnett´s post-test (n = 3; *p < 0.0001). Source data are provided as a Source Data file.
