Fig. 4: Protein kinase C is important for COL susceptibility. | Nature Communications

Fig. 4: Protein kinase C is important for COL susceptibility.

From: Colistin enhances caspofungin antifungal efficacy against Aspergillus fumigatus by modulating calcium homeostasis and stress responses

Fig. 4: Protein kinase C is important for COL susceptibility.The alternative text for this image may have been generated using AI.

A. fumigatus was grown in liquid MM using 96-well plates at 37 °C in the presence of different concentrations of enzastaurin (a) and calphostin C (b) with or without 20 μM of COL, and after 48 h, the metabolic activity (%) was assessed by the XTT assay. The results represent the average of three independent experiments ± SD and were statistically analyzed by the Two-way ANOVA test (n = 3; *****p < 0.0001, ns: not significant). c A. fumigatus wild-type and xylP::pkcA strains were grown for 96 h at 37 °C in MM+glucose 1% and MM+xylose 1 %. d A. fumigatus wild-type and xylP::pkcA strains were grown for 48 h at 37 °C in MM+glucose 1% and supplemented with increasing concentrations of xylose, in the absence or presence of 40 µM COL. Metabolic activity was evaluated by using Alamar blue. The results are expressed as the average of three independent experiments ± SD. (n = 3; Two-way ANOVA, Tukey´s post-test; *p < 0.05, **p < 0.005, **p < 0.001 and ****p < 0.0001). Source data are provided as a Source Data file.

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