Fig. 7: The calcineurin signaling pathway is important for COL bioactivity. | Nature Communications

Fig. 7: The calcineurin signaling pathway is important for COL bioactivity.

From: Colistin enhances caspofungin antifungal efficacy against Aspergillus fumigatus by modulating calcium homeostasis and stress responses

Fig. 7: The calcineurin signaling pathway is important for COL bioactivity.The alternative text for this image may have been generated using AI.

a, b A. fumigatus wild-type and ΔsltA strains were grown on solid MM without or with different treatments (COL 40 µM, CaCl2 [25, 50, or 100 mM], or a combination of COL and CaCl2). After 4 days at 37 °C, the radial growth was measured, and the values are expressed as treatment/control ratios. Results are expressed as the average of 3 independent experiments performed in technical duplicate ± SD. The data was statistically analyzed by the Two-way ANOVA test (n = 6; ****p < 0.0001 with Sidak’s multiple comparisons test comparing the ΔsltA, mutant with the wild-type strain). c, d Conidia of A. fumigatus wild-type, ΔcalA, and ΔcrzA strains (5 μL with 1 × 104 conidia) were grown on solid MM or MM supplemented with COL (20 and 40 μM), CAS (0.06 μg/mL), or combinations of both compounds, as indicated. The plates were incubated for 4 days at 37 °C. The values are expressed as treatment/control ratios and show the average of 3 independent experiments performed in technical duplicate ± SD. The data was statistically analyzed by the Two-way ANOVA test (n = 6; ****p < 0.0001 with Sidak’s multiple comparisons post-test). e A. fumigatus wild-type, ΔsltA, ΔcrzA, ΔsltA ΔcrzA, and ΔsltA:sltA+ strains (5 μL with 1 ×  104 conidia) were grown on solid MM either without any treatment or supplemented with 20 and 40 μM of COL, 0.06 μg/mL of CAS, or the combination of both, as indicated. The plates were incubated for 4 days at 37 °C. Results are expressed as treatment/control and show the average of 3 independent experiments performed in technical duplicate ± SD. The data was statistically analyzed by the Two-way ANOVA test (n = 6; ****p < 0.0001 with Tukey´s multiple comparisons post-test). f The CrzA:GFP and ΔsltA CrzA:GFP were grown for 16 h in liquid MM and transferred to CaCl2 (50 mM), COL (40 µM), and CaCl2 + COL (50 mM +  40 µM) for 15 min. CrzA:GFP nuclear translocation to the nucleus was analyzed. Hundred germlings were counted for each treatment, and the results are expressed as % of CrzA:GFP nuclear localization for germlings. Results represent the average of two independent experiments with at least 230 nuclei assessed in each assay ± SD. The data was statistically analyzed by the Two-way ANOVA test and Tukey´s post-test (****p < 0.0001, *** p < 0.0005, and * p < 0.05). Source data are provided as a Source Data file.

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