Fig. 7: HAMP1/2 overexpression in hepatocytes alleviates alcohol-induced liver injury and inflammation in Acss2-HKO mice.

a HAMP1/2 overexpression in hepatocytes was achieved by AAV-Hamp1/2 administration. AAV-Ctrl was used as control. After 2 weeks, all mice were adapted to the fluid diet by feeding the Lieber-DeCarli regular control diet for 5 consecutive days, and the model group were then fed with Lieber-DeCarli ethanol mice diet containing 5% (vol/vol) ethanol for 10 consecutive days and ethanol (5 g/kg) was administered by oral gavage on day 11 as shown in the diagram protocol was created with MedPeer (medpeer.cn). b Hepcidin levels in serum of Acss2-Flox and Acss2-HKO mice transduced with AAV-Hamp1/2 or AAV-Ctrl with EF diet (n = 5 mice per group). c Ratios of liver weight to body weight of the indicated groups of mice (n = 5 mice per group). d–f Serum ALT (d) concentrations, hepatic TG (e) and TC (f) content of the indicated groups of mice (n = 5 mice per group). g, h Representative H&E staining (g) and Oil Red O staining (h) images in liver sections from the indicated groups of mice and quantification of damaged area (n = 5 mice per group). Scale bar: 50 μm. i, j Representative images of the immunofluorescence staining and quantitative analysis of CD68 (i) and F4/80 ( j) in the liver from the indicated groups of mice in which nuclei were stained with DAPI (n = 5 mice per group). Scale bar: 50 μm. k Representative images of BODIPY 581/591 C11 staining (k), immunohistochemistry staining for 4-HNE (l), and DHE staining (m) in the liver from the indicated groups of mice (n = 5 mice per group). Scale bar: 50 μm. Quantitative analysis was also provided for each staining. n, o Hepatic MDA (n) and GSH (o) content in mice of the indicated groups (n = 5 mice per group). p, q Acetaldehyde levels in the serum and liver of the indicated groups of mice (n = 5 mice per group). Data are presented as the mean ± SD. The p-value was calculated by two-way ANOVA. Source data are provided as a Source Data file.