Fig. 2: Detection of novel class II microcin antibacterial activity by self-inhibition growth curve assay.
From: Antibacterial microcins are ubiquitous and functionally diverse across bacterial communities
A Growth curves of E. coli containing the secretion system +/− arabinose-induced expression of microcins (n = 4 per treatment). Solid lines represent the smoothed conditional means. B Area under the curve (AUC) for each growth curve in Fig. 2A. AUCs are significantly less for the induced versus uninduced treatments (p = 0.029, Wilcoxon rank-sum test, one-sided). C Growth curves of different bacterial species containing the secretion system +/− arabinose-induced expression of microcins (n = 4 per treatment). Microcins originating from species in the ‘Enterobacter’ (n = 6) and ‘Kosakonia’ (n = 1, microcin EN112) sister clades29, MccV, and empty vector negative control (NC) were secreted from Enterobacter cloacae (Ent), Leclercia adecarboxylata (Lec), and Cronobacter muytjensii (Cro). Select E. coli growth curves from Supplementary Fig. S2B and Fig. 2A are shown again for comparison. Growth curves which show self-inhibition during microcin expression (based on AUC comparisons; p = 0.029, Wilcoxon rank sum test, one-sided) are indicated with a black box.
