Fig. 2: Detailed structure of apo AmpG in inward and outward states and functional verification of contact residues. | Nature Communications

Fig. 2: Detailed structure of apo AmpG in inward and outward states and functional verification of contact residues.

From: Structural and functional insights of AmpG in muropeptide transport and multiple β-lactam antibiotics resistance

Fig. 2: Detailed structure of apo AmpG in inward and outward states and functional verification of contact residues.The alternative text for this image may have been generated using AI.

a Structure of AmpG in the inward state: top view (left) and side view (right). b The contact surface of the inward state between the N-lobe and C-lobe on the periplasmic side. c Structure of AmpG in the outward state: top view (left) and side view (right). d The contact surface of the outward state between the N-lobe and C-lobe on the cytoplasmic side. e MIC (minimum inhibitory concentration) test results for the wild type and mutations on the contact surface. blue: resistance, yellow: intermediate, red: susceptible. f TSA (thermal shift assay) results for the wild type and mutants with GlcNAc-1,6-anhMurNAc Data are shown as mean ± SD from n = 3 independent experiments.

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