Fig. 2: ENTK-instructed Tz-bearing assemblies. | Nature Communications

Fig. 2: ENTK-instructed Tz-bearing assemblies.

From: In situ construction of intracellular supramolecular assemblies as an alternative strategy for protein degradation

Fig. 2: ENTK-instructed Tz-bearing assemblies.

a Molecular structure of the self-assembled precursor Tz-MitoFlag, the corresponding hydrogelator NapFFK(G)-Tz, and SA-PROTAC. b TEM image of the nanofibers in a 300 μM NapFFK(G)-Tz hydrogel (inset). Scale bar: 200 nm. c Rheological frequency sweep of a 300 μM NapFFK(G)-Tz hydrogel. d HPLC traces used to monitor the enzymatic and bioorthogonal reactions. ENTK, 10 U/mL, 12 h. The SA-PROTAC monomer was obtained by mixing NapFFK(G)-Tz and TP2T at a 1:1 ratio at room temperature for 5 min. The TEM was repeated three times with similar results (b). Source data are provided as a Source Data file.

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