Fig. 9: Transcriptome analysis based on RNA sequencing results.
a UpSet plot and Venn map of DEGs across the saline group, RT group, and RT + PG-NSA group. b Heat map depicting the expression profiles of the top 200 DEGs across the saline, RT, and RT + PG-NSA groups. c Volcano plots comparing DEGs between saline vs. RT, RT vs. RT + PG-NSA, and saline vs. RT + PG-NSA groups, red and blue points indicate significantly upregulated and downregulated DEGs. d KEGG pathway enrichment analysis of DEGs between the RT and RT + PG-NSA groups, performed using the hypergeometric test, with bubble size proportional to DEG count and color intensity reflecting adjusted p-value (<0.05). e GO biological process enrichment analysis of DEGs between the RT and RT + PG-NSA groups, conducted using the hypergeometric test, with bar length indicating gene ratio and color intensity reflecting adjusted p-value (<0.05). f Heat map of DEGs associated with radiation-induced epithelial damage, selected based on involvement in epithelial integrity and repair pathways, across the saline, RT, and RT + PG-NSA groups. g Heat map of DEGs associated with immune response, selected based on involvement in cytokine signaling and inflammatory pathways, across the saline, RT, and RT + PG-NSA groups (n = 3 independent experiments). Abbreviations: DEGs: Differentially Expressed Genes; KEGG: Kyoto Encyclopedia of Genes and Genomes; GO: Gene Ontology.
