Table 1 Primer sequences for real-time PCR analysis

From: Tetrandrine regulates NAADP-mediated calcium signaling through a LIMP-2-dependent and sphingosine-mediated mechanism

Gene

Forward Primer

Reverse Primer

CTSC

5’-AAGGGGATCTACCACCACACT-3'

5’-GCCATAGCCCACAAGCAGAA-3'

CTSD

5’-CTGGACATCGCTTGCTGGAT-3'

5’-TGCCTCTCCACTTTGACACC-3'

GAA

5’-TCATGTTGCTGGCCAATCCA-3'

5’-CATAGGTTCCCCCACTGTTCC-3'

LGALS3BP

5’-TCTATCAGTCCAGACGGGGG-3'

5’-TCTGGATGGTGGGGAGGTAG-3'

NCSTN

5’-TTGACTGGCACAGTGGTCAA-3'

5’-ATCGTGCAGTAGAACGCACA-3'

PSAP

5’-CCCAGAGCTGGACATGACTG-3'

5’-CAAAGGTGGAGTTGGTCCGT-3'

LIMP-2

5’-GCCAATACGTCAGACAATGCCG-3'

5’-CTCATCTGCTTGGTAAAAGTGTGG-3'

SDCBP

5’-CTCTCGAAGACTTGAAGGTAGACA-3'

5’-CCATCGTGAGGGATAGGAGC-3'

TPCN1

5’-CTATGCCATCCACGACTCCC-3'

5’-ACTTGTCGTTGTTCTCGCCT-3'

TPCN2

5’-CTGGGGATTCTCAACTGCGT-3'

5’-CCAAAACCAGCAGGACAACG-3'

XBP 1

5’-CTGGAACAGCAAGTGGTAGA-3'

5’-CTGGGTCCTTCTGGGTAGAC-3'

Actin

5’-GAGAAAATCTGGCACCACACC-3'

5’-GATAGCACAGCCTGGATAGCA-3'

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